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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has long been noticed in lots of tumors, which results in chromosome instability, and so might add to tumorigenesis. This protein was located to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a vital function in promoting mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two supplemental splice variants have been reported but specific nucleotide sequence details is not really nevertheless accessible.

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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified via the antibody.

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This tutorial outlines even further attainable optimization of mobile and immunoassay parameters to be certain the absolute best effects are...

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This guide outlines further feasible optimization of cellular and immunoassay parameters to make sure the best possible success are...

Antibodies certain to the blocking peptide now not bind to the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by itself, you can see which staining is distinct.

Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Unique cyclins exhibit unique expression and degradation patterns which add into the temporal coordination of every mitotic event. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S section boundary and is also here degraded as cells progress through S section.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...

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